Copper Homeostasis in Salmonella is atypical and Copper-CueP Is a major periplasmic metal complex.

Osman, Deenah, Waldron, Kevin J, Denton, Harriet, Taylor, Clare M, Grant, Andrew J, Mastroeni, Pietro, Robinson, Nigel J and Cavet, Jennifer S (2010) Copper Homeostasis in Salmonella is atypical and Copper-CueP Is a major periplasmic metal complex. Journal of Biological Chemistry, 285 (33). pp. 25259-25268. ISSN 0021-9258

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Salmonella enterica sv. typhimurium (S. enterica sv. Typhimurium)
has two metal-transporting P1-type ATPases whose
actions largely overlap with respect to growth in elevated copper.
Mutants lacking both ATPases over-accumulate copper relative
to wild-type or either single mutant. Such duplication of
ATPases is unusual in bacterial copper tolerance. Both ATPases
are under the control of MerR family metal-responsive transcriptional
activators. Analyses of periplasmic copper complexes
identified copper-CueP as one of the predominant metal
pools. Expression of cueP was recently shown to be controlled by
the same metal-responsive activator as one of the P1-type
ATPase genes (copA), and copper-CueP is a further atypical feature
of copper homeostasis in S. enterica sv. Typhimurium. Elevated
copper is detected by a reporter construct driven by the
promoter of copA in wild-type S. enterica sv. Typhimurium during
infection of macrophages. Double mutants missing both
ATPases also show reduced survival inside cultured macrophages.
It is hypothesized that elevated copper within macrophages
may have selected for specialized copper-resistance systems
in pathogenic microorganism such as S. enterica sv.

Item Type: Article
Print ISSN: 0021-9258
Uncontrolled Keywords: Salmonella enterica sv. typhimurium (S. enterica sv. Typhimurium); bacterial copper tolerance; copper-CueP;
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life, Sport and Social Sciences
Dewey Decimal Subjects: 500 Science > 570 Life sciences; biology
Library of Congress Subjects: Q Science > QR Microbiology
Item ID: 5511
Depositing User: Dr Clare Taylor
Date Deposited: 26 Jun 2012 09:05
Last Modified: 26 Jun 2012 09:05

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