Stability of the small γ-tubulin complex requires HCA66, a protein of the centrosome and the nucleolus.

Fant, Xavier, Gnadt, Nicole, Haren, Laurence and Merdes, Andreas (2009) Stability of the small γ-tubulin complex requires HCA66, a protein of the centrosome and the nucleolus. Journal of Cell Science, 122 (8). pp. 1134-1144. ISSN 0021-9533

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    To investigate changes at the centrosome during the cell cycle,
    we analyzed the composition of the pericentriolar material
    from unsynchronized and S-phase-arrested cells by gel
    electrophoresis and mass spectrometry. We identified HCA66,
    a protein that localizes to the centrosome from S-phase to mitosis
    and to the nucleolus throughout interphase. Silencing of HCA66
    expression resulted in failure of centrosome duplication
    and in the formation of monopolar spindles, reminiscent
    of the phenotype observed after γ-tubulin silencing.
    Immunofluorescence microscopy showed that proteins of the
    γ-tubulin ring complex were absent from the centrosome in
    these monopolar spindles. Immunoblotting revealed reduced
    protein levels of all components of the γ-tubulin small complex
    (γ-tubulin, GCP2, and GCP3) in HCA66-depleted cells. By
    contrast, the levels of γ-tubulin ring complex proteins such as
    GCP4 and GCP-WD/NEDD1 were unaffected. We propose that
    HCA66 is a novel regulator of γ-tubulin function that plays a
    role in stabilizing components of the γ-tubulin small complex,
    which is in turn essential for assembling the larger γ-tubulin
    ring complex.

    Item Type: Article
    Print ISSN: 0021-9533
    Electronic ISSN: 1477-9137
    Uncontrolled Keywords: Centrosome; γ-Tubulin; Mitosis; Monopolar; Spindle;
    University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
    Dewey Decimal Subjects: 500 Science > 570 Life sciences; biology > 572 Biochemistry
    Library of Congress Subjects: Q Science > QH Natural history > QH301 Biology
    Item ID: 5484
    Depositing User: Mrs Nicole Payton
    Date Deposited: 22 Jun 2012 14:54
    Last Modified: 08 Jan 2013 11:50

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