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Improved protection against avian influenza H5N1 virus by a single microneedle skin vaccination with virus-like particles.

Song, Jei-Min, Kim, Yeu-Chun, Barlow, Peter G, Hossain, M Jaber, Park, Kyoung-Mi, Donis, Ruben O, Prausnitz, Mark R, Compans, Richard W and Kang, Sang-Moo (2010) Improved protection against avian influenza H5N1 virus by a single microneedle skin vaccination with virus-like particles. Antiviral Research, 88 (2). pp. 244-247.

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Abstract/Description

To develop a more effective vaccination method against H5N1 virus, we investigated the immunogenicity and protective efficacy after skin vaccination using microneedles coated with influenza virus-like particles containing hemagglutinin derived from A/Vietnam/1203/04 H5N1 virus (H5 VLPs). A single microneedle vaccination of mice with H5 VLPs induced increased levels of antibodies and provided complete protection against lethal challenge without apparent disease symptoms. In contrast, intramuscular injection with the same vaccine dose showed low levels of antibodies and provided only partial protection accompanied by severe body weight loss. Post-challenge analysis suggested that improved protection was associated with lower lung viral titers and enhanced generation of recall antibody secreting cells by microneedle vaccination. Thus, this study provides evidence that skin delivery of H5 VLP vaccines using microneedles designed for self-administration induces improved protection compared to conventional intramuscular immunization.

Item Type: Article
Electronic ISSN: 0166-3542
Uncontrolled Keywords: H5N1; Pandemic vaccine; Single dose; Skin vaccination; Microneedles
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Health and Social Sciences
Dewey Decimal Subjects: 600 Technology > 610 Medicine & health > 616 Diseases > 616.2 Respiratory diseases
Library of Congress Subjects: R Medicine > RF Otorhinolaryngology
Item ID: 5171
Depositing User: Mrs Lyn Gibson
Date Deposited: 27 Mar 2012 12:00
Last Modified: 06 Dec 2012 11:30
URI: http://researchrepository.napier.ac.uk/id/eprint/5171

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