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INHIBITION OF LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION BY NIPPOSTRONGYLUS BRASILIENSIS LARVAE AND EXCRETORY-SECRETORY PRODUCTS (ES).

Zhao, Min (2009) INHIBITION OF LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION BY NIPPOSTRONGYLUS BRASILIENSIS LARVAE AND EXCRETORY-SECRETORY PRODUCTS (ES). PhD thesis, Edinburgh Napier University.

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Abstract/Description

Nippostrongylus brasiliensis is a rodent intestinal nematode with an important pulmonary migrating stage. Previous studies have observed a lack of TNF-α production and minimal recruitment of neutrophils, which led us to the belief
that anti-inflammatory mechanisms could be active in the lung stage. In this study, lipopolysaccharide (LPS) stimulated alveolar macrophages (NR8383) or rat lungs were used as in vitro or in vivo inflammation models respectively.
Both live N.brasiliensis larvae and NES significantly reduced the production of LPS-induced pro-inflammatory mediators, TNF-α and NO, but not IL-1β, in NR8383 cells. The inhibition of TNF-α production was related to the heatlabile and trypsin-sensitive fraction of NES concentrate. 1-D protein gel of
NES concentrate revealed that the molecular weights of proteins are between 6kDa and 100kDa. Glycoproteins were found abundant in NES concentrate. The inflammatory processes, including NF-κB translocation and TNF-α gene
transcription were significantly inhibited by NES and/or NES concentrate. In vivo, we observed a significant reduction of neutrophil recruitment (≈ 40%) by NES on a background of LPS (100ng/ml) induced inflammation. This reduction was associated with the significant inhibition in gene transcriptions
of proinflammatory mediators TNF-α, IL-1β, iNOS, ICAM-1 and MIP-2 in bronchoalveolar lavage (BAL) cells. The down-regulation of pro-inflammatory mediators and inflammatory processes observed in this study suggests that
N.brasiliensis larvae and/or NES are capable of modifying the normally potent LPS inflammatory response, both in vitro and in vivo. This study and planned future studies could be fundamental in developing anti-inflammatory agents
with immune-active molecules in N.brasiliensis-derived products.

Item Type: Thesis (PhD)
Additional Information: APPENDIX: Zhao M, Brown DM, Maccallum J, Proudfoot L. (2009) Effect of Nippostrongylus brasiliensis L3 ES on inflammatory mediator gene transcription in lipopolysaccharide lung inflammation. Parasite Immunol. 31 50-56.
Uncontrolled Keywords: Nippostrongylus brasiliensis; Nematode worm; Secretions; Anti-inflammatory; LPS suppression;
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 600 Technology > 610 Medicine & health > 615 Pharmacology & therapeutics
500 Science > 590 Animals (Zoology) > 592 Invertebrates
500 Science > 570 Life sciences; biology > 572 Biochemistry
Library of Congress Subjects: Q Science > QL Zoology
Q Science > QH Natural history > QH301 Biology
Q Science > QP Physiology
Item ID: 2549
Depositing User: Users 10 not found.
Date Deposited: 16 Apr 2009 15:52
Last Modified: 12 Jan 2011 04:50
URI: http://researchrepository.napier.ac.uk/id/eprint/2549

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