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Lactobacillus helveticus ATCC15009 as a host for native and conjugative plasmid DNA.

Thompson, K, Collins, M A and Foley, Sophie (2002) Lactobacillus helveticus ATCC15009 as a host for native and conjugative plasmid DNA. In: Recent Research Developments in Plasmid Biology. Research Signpost/Transworld Research Network, India, pp. 55-74. ISBN 9788178950204

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Abstract/Description

The plasmid complement of Lactobacillus helveticus ATCC15009 (plasmids pLH1, pLH2 and pLH3) has been sequenced and is being analysed for functional properties. Sequences used include Accession Numbers X62150 (1-427), X72021 (21-2404 and 6891-10334), X17655 (141-2404), U83488 (260-6674), U00453 (2394-9915), L07895 (10544-12904) modified in the present laboratory. Additional sequences generated were deposited under Accession Numbers AF301605 9714-10561 and from 12905 to 14284 (unpublished). The remaining sequence was taken from Accession Number L39769 (14285-22421). All three plasmids share common sequences. Intact or degenerate IS elements are present on two of the plasmids only. It has been noted that the smallest plasmid probably encodes an element from the IS982 family of mobile genetic elements and multiple copies of this were present on the chromosome of all nine strains of Lb. helveticus tested. The use of strain ATCC15009 as a host for mobilizable recombinant plasmids derived from conjugative plasmid pIP501 is also reviewed in relation to the exploitation of this strategy to introduce new genetic material in order to study gene expression in this industrially important species.

Item Type: Book Section
Print ISSN: 81-7895-020-0
ISBN: 9788178950204
Uncontrolled Keywords: Lactbacillus helveticus; Plasmids; Sequencing; IS982; ATCC15009; pIP501;
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 500 Science > 570 Life sciences; biology > 579 Microorganisms, fungi & algae
500 Science > 570 Life sciences; biology > 576 Genetics & evolution
Library of Congress Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Item ID: 1733
Depositing User: RAE Import
Date Deposited: 17 Jul 2008 14:46
Last Modified: 02 Aug 2010 15:59
URI: http://researchrepository.napier.ac.uk/id/eprint/1733

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