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DNA binding activity of the Streptococcus thermophilus phage Sfi21 repressor.

Bruttin, Anne, Foley, Sophie and Brussow, Harald (2002) DNA binding activity of the Streptococcus thermophilus phage Sfi21 repressor. Virology, 303 (1). pp. 100-109. ISSN 0042-6822

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Abstract/Description

The cloned Streptococcus thermophilus phage Sfi21 repressor open reading frame (orf) 127 gp protects a cell against superinfection with the homologous temperate, but not against virulent phages. As demonstrated by DNase protection assay and gel shift experiments, the repressor binds to a 25-bp operator site located upstream of the repressor gene. A second sequence-related operator was identified 265 bp apart at the 3′-end of orf 75, the topological equivalent of a cro repressor gene. The replacement of a bp at the middle or at the right side of the operator decreased substantially the affinity of the repressor for the operator. In gel shift assays, the 75 gp did not bind DNA from the genetic switch region. However, when increasing amounts of orf 75 gp containing cell extracts were added to orf 127 gp containing cell extracts, the repressor could no longer bind its operator site.

Item Type: Article
Print ISSN: 0042-6822
Electronic ISSN: 1096-0341
Uncontrolled Keywords: Streptococcus thermophilus phage Sfi21 repressor; DNA binding; Binding sites; Binding suppression;
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 500 Science > 570 Life sciences; biology > 572 Biochemistry
500 Science > 570 Life sciences; biology > 579 Microorganisms, fungi & algae
500 Science > 570 Life sciences; biology > 576 Genetics & evolution
Library of Congress Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Item ID: 1732
Depositing User: RAE Import
Date Deposited: 14 Jul 2008 15:12
Last Modified: 17 Jun 2013 10:28
URI: http://researchrepository.napier.ac.uk/id/eprint/1732

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