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Evidence for the presence of an alternative glucose transport system in Clostridium beijerinckii NCIMB 8052 and the solvent hyper-producing mutant BA101.

Lee, Jieun, Mitchell , Wilfred J, Tangney, Martin and Blaschek, H P (2005) Evidence for the presence of an alternative glucose transport system in Clostridium beijerinckii NCIMB 8052 and the solvent hyper-producing mutant BA101. Applied and Environmental Microbiology, 71 (6). pp. 3384-3387. ISSN 0099 2240

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Abstract/Description

The effects of substrate analogs and energy inhibitors on glucose uptake and phosphorylation by Clostridium beijerinckii provide evidence for the operation of two uptake systems: a previously characterized phosphoenolpyruvate-dependent phosphotransferase system (PTS) and a non-PTS system probably energized by the transmembrane proton gradient. In both wild-type C. beijerinckii NCIMB 8052 and the butanol-hyperproducing mutant BA101, PTS activity declined at the end of exponential growth, while glucokinase activity increased in the later stages of fermentation. The non-PTS uptake system, together with enhanced glucokinase activity, may provide an explanation for the ability of the mutant to utilize glucose more effectively during fermentation despite the fact that it is partially defective in PTS activity.

Item Type: Article
Print ISSN: 0099 2240
Uncontrolled Keywords: Clostridium beijerinckii; Glucose uptake; Phosphotransferase system; PTS; non-PTS system;
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 500 Science > 570 Life sciences; biology > 572 Biochemistry
500 Science > 570 Life sciences; biology > 579 Microorganisms, fungi & algae
Library of Congress Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Item ID: 1727
Depositing User: RAE Import
Date Deposited: 17 Jul 2008 12:01
Last Modified: 18 Apr 2013 13:27
URI: http://researchrepository.napier.ac.uk/id/eprint/1727

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