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Flux to acetate and lactate excretions in industrial fermentations: Physiological and biochemical implications.

El-Mansi, Mansi (2004) Flux to acetate and lactate excretions in industrial fermentations: Physiological and biochemical implications. Journal of Industrial Microbiology and Biotechnology, 31 (7). 295–300. ISSN 1367 5435

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Abstract/Description

The efficiency of carbon conversion to biomass and desirable end products in industrial fermentations is diminished by the diversion of carbon to acetate and lactate excretions. In this study, the use of prototrophic and mutant strains of Escherichia coli, as well as enzyme active site directed inhibitors, revealed that flux to acetate excretion is physiologically advantageous to the organism as it facilitates a faster growth rate () and permits growth to high cell densities. Moreover, the abolition of flux to acetate excretion was balanced by the excretion of lactate as well as 2-oxoglutarate, isocitrate and citrate, suggesting a bottle-neck effect at the level of 2-oxoglutarate in the Krebs cycle. It is proposed that the acetate excreting enzymes, phosphotransacetylase and acetate kinase, constitute an anaplerotic loop or by-pass, the primary function of which is to replenish the Krebs cycle with reduced CoA, thus relieving the bottle-neck effect at the level of 2-oxoglutarate dehydrogenase. Furthermore, flux to lactate excretion plays a central role in regenerating proton gradient and maintaining the redox balance within the cell. The long-held view that flux to acetate and lactate excretions is merely a function of an over-flow in central metabolism should, therefore, be re-evaluated.

Item Type: Article
Print ISSN: 1367 5435
Electronic ISSN: 1476 5535
Uncontrolled Keywords: Acetate excretion; Phosphotransacetylase; Acetate kinase; Fluoroacetate resistance; Coenzyme A;
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 500 Science > 570 Life sciences; biology > 572 Biochemistry
500 Science > 570 Life sciences; biology > 579 Microorganisms, fungi & algae
Library of Congress Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Item ID: 1721
Depositing User: RAE Import
Date Deposited: 14 Jul 2008 09:33
Last Modified: 10 May 2013 11:16
URI: http://researchrepository.napier.ac.uk/id/eprint/1721

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