A comparison of five rapid direct toxicity assessment methods to determine toxicity of pollutants to activated sludge.

Dalzell, David, Alte, S, Aspichueta, Elena, De la Sota, Alejandro, Etxebarria, J, Gutierrez, M, Hoffmann, C, Sales, D, Obst, U and Christofi, Nicholas (2005) A comparison of five rapid direct toxicity assessment methods to determine toxicity of pollutants to activated sludge. Chemosphere, 47 (5). pp. 535-545. ISSN 0045 6535

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Five rapid direct toxicity assessment methods were used in three European partner countries to determine the toxicity of single toxicants, mixed toxicants and real industrial wastes. The final aim was to protect microbial degradation of organic wastes in biological treatment processes and hence enhance the quality of treated effluents to be discharged to the environment. Nitrification inhibition, Respirometry, Adenosine triphosphate luminescence and Enzyme inhibition were tested utilising activated sludge as the testing matrix. The Vibrio fischeri toxicity test was used as a surrogate to compare the various microbial bioassays. The IC50 (toxicant concentration eliciting a 50% inhibitory effect) was determined for a number of pollutants including single toxicants Cd, Cr, Cu, Zn, 3,5-dichlorophenol, toluene and linear alkylbenzenesulphonate (LAS); a standard mixture of metals and LAS; a standard mixture of organics and LAS, and 16 industrial effluents. The V. fischeri bioassay was also chosen in order to assess quality control of toxicant preparation during testing in the different laboratories of the partner countries. Comparisons of sensitivity, cost of implementation, cost per test, relevance, and ease of use were made. The most sensitive bioassays were V. fischeri and Nitrification inhibition, however, this depended in the main on the pollutant and mixtures tested. It is recommended that during assessment of wastewater toxicity a suite of tests be used rather than reliance on one particular test.

Item Type: Article
Print ISSN: 0045 6535
Uncontrolled Keywords: Activated sludge; Toxicity bioassays; Nitrification inhibition; Respirometry; ATP luminescence; Enzyme; Vibrio fischeri
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 500 Science > 570 Life sciences; biology > 572 Biochemistry
500 Science > 570 Life sciences; biology > 579 Microorganisms, fungi & algae
Library of Congress Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Item ID: 1719
Depositing User: RAE Import
Date Deposited: 14 Jul 2008 10:27
Last Modified: 18 Apr 2013 12:35

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