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Reduced alveolar macrophage migration induced by acute ambient particle (PM10) exposure.

Barlow, Peter G, Brown, David M, Donaldson, Ken, McCallum, Janis and Stone, Vicki (2008) Reduced alveolar macrophage migration induced by acute ambient particle (PM10) exposure. Cell Biology and Toxicology, 24 (3). pp. 243-252. ISSN 0742-2091

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Abstract/Description

Increased levels of particulate air pollution (PM10) have been implicated as a causal agent in pulmonary disease exacerbation and increased deaths from respiratory and cardiovascular disorders. The exact mechanism by which PM10 drives toxicity in the lung is still unknown, but studies have focused on inhibition of macrophage function and impaired alveolar clearance mechanisms. To assess the effects of PM10 on pulmonary macrophage clearance mechanisms ex vivo, Wistar rats were instilled with 125 or 250 μg of PM10 collected from the North Kensington, London. Control rats were instilled with sterile saline. The rats were sacrificed after 18 h and a bronchoalveolar lavage (BAL) was performed. Macrophages isolated from the BAL fluid were assessed for ability to migrate towards a positive chemoattractant (ZAS) ex vivo and to perform phagocytosis. Macrophages isolated from the PM10-exposed rats showed inhibition of potential to migrate. Macrophage phagocytic ability ex vivo was also significantly reduced by the presence of PM10 inside the cells. This study indicates that acute PM10 exposure diminishes macrophage motility and phagocytosis in a manner that could prove deleterious to particle clearance from the alveolar region of the lung. Decreased particle clearance promotes inflammation, and hence, warrants further investigation in relation to the effects of chronic PM10 exposure on macrophage clearance mechanisms and establishing the mechanisms behind decreased macrophage migration.

Item Type: Article
Print ISSN: 0742-2091
Electronic ISSN: 1573-6822
Uncontrolled Keywords: Air pollution; PM10; Effects; Macrophages; Lung function; Phagocytosis; Chemotaxis;
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 600 Technology > 610 Medicine & health > 612 Human physiology
600 Technology > 620 Engineering > 620 Engineering & allied operations
600 Technology > 610 Medicine & health > 616 Diseases
Library of Congress Subjects: Q Science > QD Chemistry
Q Science > QP Physiology
R Medicine > RC Internal medicine
Item ID: 1676
Depositing User: RAE Import
Date Deposited: 27 Jun 2008 12:29
Last Modified: 11 Feb 2011 14:55
URI: http://researchrepository.napier.ac.uk/id/eprint/1676

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