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Inflammation-associated gene expression is altered between normal human ovarian surface epithelial cells and cell lines derived from ovarian adenocarcinomas.

Gubbay, O, Guo, W, Rae, Mick, Niven, D, Langdon, S P and Hillier, S G (2005) Inflammation-associated gene expression is altered between normal human ovarian surface epithelial cells and cell lines derived from ovarian adenocarcinomas. British Journal of Cancer, 92 (10). pp. 1927-33. ISSN 0007 0920

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Abstract/Description

Ovulation is believed to contribute to the development of ovarian cancers that derive from the ovarian surface epithelium (OSE). The process of ovulation is synonymous with inflammation and inflammatory cytokines such as interleukin-1α (IL-1α) have recently been shown to induce both inflammatory and anti-inflammatory responses in human OSE (HOSE) cells. In this study we directly compared levels of IL-1α-induced gene expression by analysing the levels of I I /?-hydroxysteroid dehydrogenase (11βHSD) types I (I IβHSD-1) and 2 (IIβHSD-2), cyclooxygenase-2 (COX-2), IL-I receptor (IL-IR) and glucocorticoid receptor α (GRα) mRNA between normal HOSE cells and cell lines derived from poorly differentiated (SKOV-3, BG-1, PEO-4) and well-differentiated (PEO-14) ovarian adenocarcinoma. In HOSE cell cultures, and to a lesser extent PEO-14 cells, the basal mRNA levels of COX-2 and I 1βHSD-1 were relatively high and further shown to be induced in response to IL-1α (for HOSE cells; >20-fold, P<0.05 and PEO-14 cells; >3fold, P<0.05). However, whereas HOSE cells expressed a low level of I 1βHSD-2 mRNA that was only mildly responsive to IL-1α (1,3-fold, P<0.001), all cell lines exhibited a higher basal level of I 1βHSD-2 mRNA that was in some cases further stimulated in PEO-4 cells (five-fold; P<0.05) or suppressed in SKOV-3 cells (two-fold; P<0.01) in response to IL-1α. All cells tested expressed IL-1R and, with the exception of BG-1, GRα. These results indicate that cell lines derived from ovarian cancers have lost the ability to respond normally to inflammatory cytokines such as IL-1α. The finding that normal OSE cells, in contrast to cell lines derived from patients with ovarian adenocarcinoma, abundantly express I 1βHSD-1 mRNA but are essentially devoid of I 1βHSD-2 mRNA supports the concept that the pattern of I 1βHSD isoform gene expression is a defining feature of neoplastic cellular transformation, which might have particular relevance to the ovary.

Item Type: Article
Print ISSN: 0007 0920
Electronic ISSN: 1532-1827
Uncontrolled Keywords: Malignant tumor ; Female genital diseases ; Female genital system ; Ovarian diseases ; Ovary adenocarcinoma ; Cancerology ; Epithelium ; Established cell line ; Epithelial cell ; Cell surface ; Human ; Gene expression ; Inflammation ; Ovary cancer ;
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 500 Science > 570 Life sciences; biology > 572 Biochemistry
600 Technology > 610 Medicine & health > 618 Gynecology, obstetrics, pediatrics & geriatrics
600 Technology > 610 Medicine & health > 611 Human anatomy, cytology & histology
Library of Congress Subjects: R Medicine > RG Gynecology and obstetrics
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Q Science > QR Microbiology
Item ID: 1663
Depositing User: RAE Import
Date Deposited: 30 Jun 2008 11:24
Last Modified: 18 Apr 2013 13:38
URI: http://researchrepository.napier.ac.uk/id/eprint/1663

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