Antiinflammatory steroid action in human ovarian surface epithelial cells.

Rae, Mick T, Niven, D, Critchley, Hilary O D and Harlow, C R (2004) Antiinflammatory steroid action in human ovarian surface epithelial cells. Journal of Clinical Endocrinology and Metabolism, 89 (9). pp. 4538-4544. ISSN 0021-972X

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The human ovarian surface epithelium (OSE) is subject to
serial injury and repair during ovulation, which is a natural inflammatory event. We asked whether there is a compensatory antiinflammatory component to this process, involving steroid hormones produced locally at the time of ovulation. Quantitative RT-PCR analysis of total RNA from cultured human OSE cell monolayers showed that exposure to proinflammatory IL1Alpha (500 pg/ml) increased mRNA levels of cyclooxygenase-2 (COX-2) (P < 0.01) at 48 h. The COX-2mRNAresponse to IL1Alpha was associated with an approximate 18-fold (P < 0.01) increase in mRNA levels of 11Beta-hydroxysteroid dehydrogenase type 1 (11BetaHSD1), encoding the steroid dehydrogenase that reversibly reduces cortisone to antiinflammatory cortisol. Addition of cortisol to OSE cell culture medium dosedependently
suppressed the COX-2 mRNA response to IL1Alpha (P < 0.01) but reciprocally enhanced the 11BetaHSD1 mRNA response
(P < 0.05), with both effects strongest at 1 muM cortisol.
Presence of glucocorticoid receptor-Alpha mRNA and protein was established in OSE cell monolayers and treatment with IL1Alpha shown to significantly up-regulate the glucocorticoid receptor- Alpha mRNA level (P < 0.05). Glucocorticoid receptor antagonist (RU486, 10 muM) fully reversed the inhibitory effect of 1 muM cortisol on IL1Alpha-stimulated COX-2 mRNA expression. Progesterone also suppressed IL1Alpha-induced COX-2mRNAexpression but had no significant effect on IL1Alpha-stimulated 11BetaHSD1 expression. These data provide direct evidence for antiinflammatory actions of cortisol and progesterone in human OSE cells.

Item Type: Article
Print ISSN: 0021-972X
Electronic ISSN: 1945-7197
Uncontrolled Keywords: Humans; Ovaries; Epithelium; Anti-inflammatory; Steroids; Biochemistry;
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 500 Science > 570 Life sciences; biology > 572 Biochemistry
600 Technology > 610 Medicine & health > 615 Pharmacology & therapeutics
Library of Congress Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
R Medicine > RM Therapeutics. Pharmacology
Item ID: 1661
Depositing User: RAE Import
Date Deposited: 25 Jun 2008 11:19
Last Modified: 22 Oct 2014 15:44

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