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Anti-inflammatory responses and oxidative stress in Nippostrongylus brasiliensis-induced pulmonary inflammation.

McNeil, Kathryn S, Knox, David P and Proudfoot, Lorna (2002) Anti-inflammatory responses and oxidative stress in Nippostrongylus brasiliensis-induced pulmonary inflammation. Parasite Immunology, 24 (1). pp. 15-22. ISSN 0141-9838

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Abstract/Description

Migration of L3 larvae of Nippostrongylus brasiliensis through the lungs of the rat, during primary infection, was studied at 24 h, 72 h and 8 days. At 24 h p.i., there was evidence of damage to lung epithelial cells and microvasculature, with increased protein and γ-glutamyl transpeptidase in the bronchoalveolar lavage (BAL) fluid. However, there was little evidence of inflammatory cell recruitment. At 24 h p.i., there was a significant reduction in the inflammatory cytokine tumour necrosis factor α. Superoxide (O2−·) production was also reduced, accompanied by an increase in superoxide dismutase activity. Lipid peroxidation was reduced at 24 h p.i. and L3 larvae were shown to possess high levels of glutathione compared to host lung tissue. Nitric oxide, detected as nitrite, was produced in BAL fluid, and inducible nitric oxide synthase protein was increased by 72 h p.i. There was evidence of peroxynitrite production throughout the infection period with specific protein bands nitrosylated at 75, 30 and 25 kDa. It appears that despite early evidence of lung damage, the inflammation was reduced in response to L3 larvae of N. brasiliensis.

Item Type: Article
Print ISSN: 0141-9838
Electronic ISSN: 1365-3024
Uncontrolled Keywords: Nippostrongylus brasiliensis; Bronchoalveolar lavage; Pulmonary responses; Oxidative stress; Nitric oxide
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 600 Technology > 610 Medicine & health > 615 Pharmacology & therapeutics
600 Technology > 610 Medicine & health > 612 Human physiology
600 Technology > 610 Medicine & health > 611 Human anatomy, cytology & histology
600 Technology > 610 Medicine & health > 614 Incidence & prevention of disease
Library of Congress Subjects: Q Science > QP Physiology
Q Science > QM Human anatomy
Item ID: 1647
Depositing User: RAE Import
Date Deposited: 23 Jun 2008 15:31
Last Modified: 02 Aug 2010 16:34
URI: http://researchrepository.napier.ac.uk/id/eprint/1647

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