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The effects of PM10 particles and oxidative stress on macrophages and lung epithelial cells: modulating effects of calcium-signaling antagonists.

Brown, David M, Hutchison, Laura, Donaldson, Ken and Stone, Vicki (2007) The effects of PM10 particles and oxidative stress on macrophages and lung epithelial cells: modulating effects of calcium-signaling antagonists. American Journal of Physiology. Lung Cellular and Molecular Physiology, 292 (6). L1444-L1451. ISSN 1522-1504

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Abstract/Description

We have previously examined the ability of air pollution particles (PM10) to promote release of the proinflammatory cytokine tumor necrosis factor- (TNF-) from human peripheral blood mononuclear cells and demonstrated a role for calcium as a signaling molecule in this process. We have now studied the ability of oxidative stress induced by a synthetic oxidant tert-butyl hydroperoxide (tBHP) to induce TNF- production via calcium signaling in the mouse macrophage cell line (J774). The oxidant tBHP significantly increased intracellular calcium and the release of TNF- in J774 cells, an effect that was reduced to control levels by inhibition of calcium signaling with verapamil, BAPTA-AM, and W-7. This study also investigated interactions between PM10-treated macrophages and epithelial cells by using conditioned medium (CM) from PM10-treated mononuclear cells to stimulate the release of the neutrophil chemoattractant chemokine IL-8 from A549 lung epithelial cells. TNF- protein release was demonstrated in human mononuclear cells after PM10 treatment, an effect that was inhibited by calcium antagonists. Treatment of A549 cells with monocyte/PM10 CM produced increased IL-8 release that was reduced with CM from monocyte/PM10/calcium antagonist treatments. The expression of ICAM-1 was increased after incubation with CM from monocyte/PM10 treatment, and this increase was prevented by treatment with CM from monocyte/PM10/calcium antagonist. These data demonstrate a link between oxidative stress, calcium, and inflammatory mediator production in macrophages and lung epithelial cells.

Item Type: Article
Print ISSN: 1522-1504
Electronic ISSN: 1040-0605
Uncontrolled Keywords: Lung macrophages; Cytokines
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 600 Technology > 610 Medicine & health > 612 Human physiology
600 Technology > 610 Medicine & health > 611 Human anatomy, cytology & histology
600 Technology > 610 Medicine & health > 614 Incidence & prevention of disease
Library of Congress Subjects: Q Science > QP Physiology
Item ID: 1628
Depositing User: RAE Import
Date Deposited: 05 Jun 2008 10:25
Last Modified: 21 Jul 2010 13:37
URI: http://researchrepository.napier.ac.uk/id/eprint/1628

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