INSPIRING FUTURES

High-intensity exercise elicits the mobilization of senescent T lymphocytes into the peripheral blood compartment in human subjects.

Simpson, Richard J, Florida-James, Geraint, Cosgrove, Cormac, Whyte, Greg P, Macrae, Scott, Pircher, Hanspeter and Guy, Keith (2007) High-intensity exercise elicits the mobilization of senescent T lymphocytes into the peripheral blood compartment in human subjects. Journal of Applied Physiology, 103 (1). pp. 396-401. ISSN 15221601

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Abstract/Description

Clonal expansion of T lymphocytes in response to antigenic stimulation is a fundamental process of adaptive immunity. As a consequence of clonal expansion, some T lymphocytes acquire a senescent phenotype, fail to replicate in response to further antigenic stimulation, and express the killer cell lectin-like receptor G1 (KLRG1) and/or CD57. Physical exercise elicits a mobilization of large numbers of T lymphocytes into the bloodstream from peripheral lymphoid compartments, but the frequency of senescent cells in the mobilized population is not known. Eight male runners (age: 29 ± 9 yr; maximal O2 uptake 62 ± 6 ml·kg–1·min–1) performed an intensive treadmill-running protocol at 80% maximal O2 uptake to volitional exhaustion. Blood lymphocytes isolated before, immediately after, and 1 h after exercise were assessed for cell surface expression of KLRG1, CD57, CD28, CD45RA, CD45RO, CD62L, and lymphocyte subset markers (CD3, CD4, CD8, CD56) by flow cytometry. The percentage of all CD3+ T lymphocytes expressing KLRG1 and CD57 increased with exercise (P < 0.01). The change in T-lymphocyte KLRG1 expression was attributed to both CD4+ and CD8 bright T cells, with the relative change being greater for the CD8 bright population (P < 0.01). Mobilized T-lymphocyte populations expressing KLRG1 and CD57 appeared to extravasate the peripheral blood compartment after 1 h of recovery. In conclusion, T lymphocytes with a senescent phenotype are mobilized and subsequently removed from the bloodstream in response to acute high-intensity exercise. This suggests that T lymphocytes contained within the peripheral lymphoid compartments that are mobilized by exercise are likely to be at a more advanced stage of biological aging and have a reduced capacity for clonal expansion than blood-resident T cells.

Item Type: Article
Print ISSN: 15221601
Uncontrolled Keywords: T lymphocytes; Antigenic stimulation; Adaptive immunity; Clonal expansion; Senescent phenotype; Killer cell lectin-like receptor; Flow cytometry; Lymphocyte trafficking
University Divisions/Research Centres: Faculty of Health, Life & Social Sciences > School of Life Sciences
Dewey Decimal Subjects: 500 Science > 570 Life sciences; biology > 572 Biochemistry
500 Science > 570 Life sciences; biology > 571 Physiology & related subjects
600 Technology > 610 Medicine & health > 612 Human physiology
Library of Congress Subjects: Q Science > QP Physiology
Q Science > QR Microbiology > QR180 Immunology
Item ID: 1623
Depositing User: RAE Import
Date Deposited: 11 Feb 2008 10:35
Last Modified: 18 Feb 2013 16:22
URI: http://researchrepository.napier.ac.uk/id/eprint/1623

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