Barlow, Peter G, Clouter-Baker, Anna, Donaldson, Ken, MacCallum, Janis and Stone, Vicki (2005) Carbon black nanoparticles induce type II epithelial cells to release chemotaxins for alveolar macrophages. Particle and Fibre Toxicology, 2. pp. 11-24. ISSN 1743-8977Full text not available from this repository. (Request a copy)
Alveolar macrophages are a key cell in dealing with particles deposited in the lungs and in determining the subsequent response to that particle exposure. Nanoparticles are considered a potential threat to the lungs and the mechanism of pulmonary response to nanoparticles is currently under intense scrutiny. The type II alveolar epithelial cell has previously been shown to release chemoattractants which can recruit alveolar macrophages to sites of particle deposition. The aim of this study was to assess the responses of a type II epithelial cell line (L-2) to both fine and nanoparticle exposure in terms of secretion of chemotactic substances capable of inducing macrophage migration.
Exposure of type II cells to carbon black nanoparticles resulted in significant release of macrophage chemoattractant compared to the negative control and to other dusts tested (fine carbon black and TiO2 and nanoparticle TiO2) as measured by macrophage migration towards type II cell conditioned medium. SDS-PAGE analysis of the conditioned medium from particle treated type II cells revealed that a higher number of protein bands were present in the conditioned medium obtained from type II cells treated with nanoparticle carbon black compared to other dusts tested. Size-fractionation of the chemotaxin-rich supernatant determined that the chemoattractants released from the epithelial cells were between 5 and 30 kDa in size.
The highly toxic nature and reactive surface chemistry of the carbon black nanoparticles has very likely induced the type II cell line to release pro-inflammatory mediators that can potentially induce migration of macrophages. This could aid in the rapid recruitment of inflammatory cells to sites of particle deposition and the subsequent removal of the particles by phagocytic cells such as macrophages and neutrophils. Future studies in this area could focus on the exact identity of the substance(s) released by the type II cells in response to particle exposure.
|Uncontrolled Keywords:||Alveolar macrophages; Nanoparticles; Pulmonary response; Chemoattractants;|
|University Divisions/Research Centres:||Faculty of Health, Life & Social Sciences > School of Life Sciences|
|Dewey Decimal Subjects:||500 Science > 570 Life sciences; biology > 572 Biochemistry|
600 Technology > 610 Medicine & health > 615 Pharmacology & therapeutics
600 Technology > 620 Engineering > 620 Engineering & allied operations
600 Technology > 610 Medicine & health > 616 Diseases
|Library of Congress Subjects:||Q Science > QR Microbiology|
|Depositing User:||RAE Import|
|Date Deposited:||11 Feb 2008 10:10|
|Last Modified:||03 Apr 2013 14:02|
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